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In vitro arrow cane (Gynerium sagitatum Aubl.) multiplication in double phase medium

Lopez D., Claudia Marcela Y Suarez P., Isidro Elias (2018) In vitro arrow cane (Gynerium sagitatum Aubl.) multiplication in double phase medium. Revista de Ciencias Agrícolas, 35 (2). pp. 5-13. ISSN 2256-2273

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Arrow cane (Gynerium sagitatum Aubl.) is a Poaceae species used as fiber source to make traditional and valuable handmade craftsmanship by indigenous communities in Northern Colombia. Since no commercial crops are established fiber needs are taken from natural plant populations affecting ecosystem. A micropropagation protocol to clonally multiply large quantities of arrow cane plant material for planting commercial crops has been developed; however, micropropagated plants are costly compared to naturally extracted plant material. To reduce micropropagated plants costs, in the present research a double phase medium formulation along with continuous shoot culture with no periodic transfers to fresh medium was compared to semisolid medium system with subculture every four weeks with respect to multiplication rate and costs of micropropagated plants. The results showed that continuous culture of explants with double phase medium and no periodic transfers resulted in higher multiplication rates and larger shoots compared to shoots cultured using the conventional semisolid medium system and transfer to fresh medium every four weeks. Plants from both, semisolid and double phase culture system, fully adapted and recovered when transferred to ex vitro conditions. The cost analysis showed that double phase cultured shoots are ≥20% less expensive.

Tipo de Elemento: Artículo
Palabras Clave: Propagación in vitro, doble fase, meristemos axilares, BAP, ex vitro
Asunto: S Agricultura > S Agriculture (General)
Division: Revistas > Revista de Ciencias Agrícolas
Depósito de Usuario: FACIA Fac. Ciencias Agrícolas Udenar
Fecha Deposito: 01 Oct 2021 20:28
Ultima Modificación: 01 Oct 2021 20:28

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