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Estandarización del protocolo para evaluar la actividad enzimática de peroxidasa en tomate de arbol (solanum betaceum cav.)

Santander, Margareth Y Mejía, Diego Y Osorio M., Oswaldo (2013) Estandarización del protocolo para evaluar la actividad enzimática de peroxidasa en tomate de arbol (solanum betaceum cav.). Temas Agrarios, 18 (2). pp. 36-49. ISSN 0122-7610

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Resumen

The aim of this research was to obtain an analysis protocol of the enzymatic activity of Peroxidase (POD) in tree tomato pulp (Solanum betaceum Cav.). Variables were evaluated in the extraction process as the percentage of pulp (PP), of 20 to 50% and the centrifugation time (CT) of 10 to 30 min, while in the measurement was varied extract volume (EV) of 30 to 90 L. It was used an experimental central composite design 23 more star points and center points with response surface methodology replicated twice for a total of 32 experimental units. For optimization, the highest enzyme activity is presented using a PP of 60%, a CT of 34 min and a VE of 110 μl.

Tipo de Elemento: Artículo
Palabras Clave: Enzimes, peroxidase activity, fruit, pulp, protocol Enzimas, actividad peroxidasa, frutales, pulpa, protocolo
Asunto: Q Ciencias > QD Chemistry
S Agricultura > S Agriculture (General)
Division: Facultad de Ingeniería Agroindustrial > Programa de Ingeniería Agroindustrial > Productividad
Depósito de Usuario: ING AI DIEGO FERNANDO MEJIA ESPAÑA
Fecha Deposito: 31 Jan 2017 20:33
Ultima Modificación: 18 Dec 2017 22:11
URI: http://sired.udenar.edu.co/id/eprint/3614

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