Detection of auxinic compounds in germinating seedlings

Oliva-Hernández, Amanda Alejandra Y Quiroz-Velásquez, Jesús Di Carlo Y García-Olivares, Jesus Gerardo Y García-León, Israel Y Lizarazo-Ortega, Cristian Y Hernández-Mendoza, Jose Luis (2021) Detection of auxinic compounds in germinating seedlings. Revista de Ciencias Agrícolas, 38 (2). pp. 63-74. ISSN 2256-2273

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Resumen

Tryptophan (TRP) is a metabolite from which several important metabolic syntheses arise in plants, animals, and humans. In bacteria and fungi, it is a precursor of Indole Acetic acid (IAA) using various metabolic pathways. The objective of this study is the detection of intermediate metabolites in the synthesis of IAA in seeds of several species in the germination process. In the study, seeds of plant species grown in deionized water were placed in order to stimulate germination and samples were taken every 24 hours. High performance liquid chromatography (HPLC) was used for the detection of the compounds. The results show that the pH of the medium is altered and there is no pattern of behavior. Regarding the detected compounds, in addition to TRP, there is indole-3-acetamide (IAM), 3-indoleacetonitrile (IAN), tryptamine (TRM), which are part of the TRP-dependent routes, since they use this amino acid as a precursor. Anthranilic acid (AA) and kynurenine (KYN), which are part of the Independent TRP pathway, were also detected. IAA and TRP were also detected during the germination process of the studied seeds (Sorghum bicolor, T aesativum, Zea mayz, Phaseolus vulgaris, G. hirsutum, Cucurbita maxima). Finally, it was observed that the seeds, due to weight loss, suffer physical wear during the germination process, since there is a difference between the initial dry weight and the weight of the seeds at the end of the study.

Tipo de Elemento:	Artículo
Palabras Clave:	Kynurenine, pH, IAA pathway, growth-promoting substances, HPLC
Asunto:	S Agricultura > S Agriculture (General)
Division:	Revistas > Revista de Ciencias Agrícolas
Depósito de Usuario:	FACIA Fac. Ciencias Agrícolas Udenar
Fecha Deposito:	08 Dec 2021 17:35
Ultima Modificación:	08 Dec 2021 17:35
URI:	http://sired.udenar.edu.co/id/eprint/7246

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